Organization of Escherichia coli O157 O Antigen Gene Cluster and Identification of Its Specific Genes
نویسندگان
چکیده
منابع مشابه
Organization of Escherichia coli O157 O antigen gene cluster and identification of its specific genes.
The O157:H7 clone of Escherichia coli, which causes major, often prolonged outbreaks of gastroenteritis with hemolytic-uremic syndrome (HUS) such as those in Japan, Scotland, and the United States recently, is thought to be resident normally in cattle or other domestic animals. This clone is of major significance for public health and the food industry. We have developed a fast method for seque...
متن کاملSequencing of Escherichia coli O111 O-antigen gene cluster and identification of O111-specific genes.
Shiga toxin (Stx)-producing Escherichia coli strains of serogroup O111 are the most frequently isolated non-O157 strains causing outbreaks of gastroenteritis with hemolytic-uremic syndrome. The O111 O-antigen gene cluster had been cloned and about half of it has been sequenced; we have now sequenced the remainder of the gene cluster, which is 12.5 kb in length and which comprises 11 genes. On t...
متن کاملCharacterization of Escherichia coli O86 O-antigen gene cluster and identification of O86-specific genes.
Escherichia coli O86 belongs to the enteropathogenic E. coli (EPEC) group, some strains of which are pathogens of humans, wild birds and farm animals. The O-antigen gene cluster of E. coli O86 was amplified by long-range PCR using primers based on the housekeeping genes galF and gnd, and then sequenced. Genes involved in GDP-Fuc and N-acetyl-galactosamine (GalNAc) synthesis and genes encoding g...
متن کاملIdentification of Escherichia coli O172 O-antigen gene cluster and development of a serogroup-specific PCR assay.
AIM To characterize the locus for O-antigen biosynthesis from Escherichia coli O172 type strain and to develop a rapid, specific and sensitive PCR-based method for identification and detection of E. coli O172. METHODS AND RESULTS DNA of O-antigen gene cluster of E. coli O172 was amplified by long-range PCR method using primers based on housekeeping genes galF and gnd Shot gun bank was constru...
متن کاملEscherichia coli O157 Cluster Evaluation
We investigated a multistate cluster of Escherichia coli O157:H7 isolates; pulsed-field gel electrophoresis subtyping, using a single enzyme, suggested an epidemiologic association. An investigation and additional subtyping, however, did not support the association. Confirmating E. coli O157 clusters with two or more restriction endonucleases is necessary before public health resources are allo...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1998
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.66.8.3545-3551.1998